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xanthoproteic reaction
The xanthoproteic reaction is a method that can be used to determine the amount of protein soluble in a solution, using concentrated nitric acid. The test gives a positive result in those proteins with amino acids carrying aromatic groups, especially in the presence of tyrosine. If the test is positive the proof is neutralized with an alkali, turning dark yellow. The yellow colour is due to xanthoproteic acid which is formed due to nitration of certain amino acids, most common examples being tyrosine and tryptophan. This chemical reaction is a qualitative test, determining the presence or absence of proteins.
== Procedure ==
Add 1 ml of Concentrated HNo3 to 1 ml of the test sample. Heat the mixture and cooled it. Slowly add sodium hydroxide (40% w/v in water) solution till the mixture become alkaline and color changes is noted. If color changes from yellow to Orange. It indicates the presence of aromatic amino acid.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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